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Number of mismatches (bp) observed in gap sequence of merged reads across circular and linear Nicking Loop™ workflows when compared to the expected reference gap sequence. Circular library was sequenced by DNBSEQ™-G99 and linear library was sequenced with Illumina MiSeq. Reads obtained by DNBSEQ™-G99 were subsampled without replacement to match the sequencing depth of Illumina MiSeq.
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Number of mismatches (bp) observed in gap sequence of merged reads across circular and linear Nicking Loop™ workflows when compared to the expected reference gap sequence. Circular library was sequenced by DNBSEQ™-G99 and linear library was sequenced with Illumina MiSeq. Reads obtained by DNBSEQ™-G99 were subsampled without replacement to match the sequencing depth of Illumina MiSeq.
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Journal: bioRxiv

Article Title: Application of the Nicking Loop™ targeted library preparation method to DNBSEQ™ sequencing

doi: 10.64898/2026.03.10.710732

Figure Lengend Snippet: Number of mismatches (bp) observed in gap sequence of merged reads across circular and linear Nicking Loop™ workflows when compared to the expected reference gap sequence. Circular library was sequenced by DNBSEQ™-G99 and linear library was sequenced with Illumina MiSeq. Reads obtained by DNBSEQ™-G99 were subsampled without replacement to match the sequencing depth of Illumina MiSeq.

Article Snippet: Nicking LoopTM libraries were amplified into DNA nanoballs (DNBs) and sequenced on the DNBSEQTM-G99 platform following the manufacturer’s system guide (Complete Genomics, San Jose, CA).

Techniques: Sequencing